Copper amine oxidases (CuAOs) involved in polyamine catabolism are emerging as physiologically relevant enzymes for their involvement in plant growth, differentiation and defence responses to biotic and abiotic stress. In this chapter, we describe two spectrophotometric and one polarographic method for determining CuAO activity in plant tissues. Some aspects related to cell wall association of apoplastic CuAOs and possible interference of plant metabolites with the enzymatic activity assays are also considered.
Angelini, R., Cona, A., Tavladoraki, P. (2018). Determination of copper amine oxidase activity in plant tissues. In T.A.F. Alcazar Ruben (a cura di), "Polyamines" - Methods in Molecular Biology (pp. 129-139). New York : Humana Press Inc. [10.1007/978-1-4939-7398-9_13].
Determination of copper amine oxidase activity in plant tissues
Angelini, Riccardo;Cona, Alessandra;Tavladoraki, Paraskevi
2018-01-01
Abstract
Copper amine oxidases (CuAOs) involved in polyamine catabolism are emerging as physiologically relevant enzymes for their involvement in plant growth, differentiation and defence responses to biotic and abiotic stress. In this chapter, we describe two spectrophotometric and one polarographic method for determining CuAO activity in plant tissues. Some aspects related to cell wall association of apoplastic CuAOs and possible interference of plant metabolites with the enzymatic activity assays are also considered.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
