Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-gamma) in the presence of anti-IFN-beta antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-gamma in FLC is in part mediated by the production of IFN-beta. Accordingly, IFN-gamma induces a less pronounced antiviral state in FLC resistant to IFN-alpha/beta than in wild-type cells. Moreover, while results of run-on assays indicate that both IFN-alpha and -beta genes are constitutively transcribed in these cells, FLC treatment with IFN-gamma induces only IFN-beta mRNA accumulation. These results indicate that posttranscriptional mechanisms are involved in the regulation of IFN-beta and -alpha expression by IFN-gamma. The low amounts of the induced IFN-beta synergize with IFN-gamma in mounting the potent antiviral effect.

Marziali, G., Fiorucci, G., Coccia, E.M., Percario, Z., Raber, J., Battistini, A., et al. (1991). Post-transcriptional regulation of interferon-beta expression in erythroid Friend cells treated with interferon-gamma. JOURNAL OF VIROLOGY, 65(8), 4130-4136.

Post-transcriptional regulation of interferon-beta expression in erythroid Friend cells treated with interferon-gamma

AFFABRIS, Elisabetta;
1991-01-01

Abstract

Treatment of Friend erythroleukemia cells (FLC) with gamma interferon (IFN-gamma) in the presence of anti-IFN-beta antibodies reduces the effectiveness of the antiviral state and the induction of 2'-5'-oligoadenylate synthetase activity, indicating that the antiviral activity of IFN-gamma in FLC is in part mediated by the production of IFN-beta. Accordingly, IFN-gamma induces a less pronounced antiviral state in FLC resistant to IFN-alpha/beta than in wild-type cells. Moreover, while results of run-on assays indicate that both IFN-alpha and -beta genes are constitutively transcribed in these cells, FLC treatment with IFN-gamma induces only IFN-beta mRNA accumulation. These results indicate that posttranscriptional mechanisms are involved in the regulation of IFN-beta and -alpha expression by IFN-gamma. The low amounts of the induced IFN-beta synergize with IFN-gamma in mounting the potent antiviral effect.
Marziali, G., Fiorucci, G., Coccia, E.M., Percario, Z., Raber, J., Battistini, A., et al. (1991). Post-transcriptional regulation of interferon-beta expression in erythroid Friend cells treated with interferon-gamma. JOURNAL OF VIROLOGY, 65(8), 4130-4136.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11590/121129
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