Resveratrol, a stilbene found in grapes and wine, is one of the most interesting natural compound due to its role exerted in cancer prevention and therapy. In particular, resveratrol is able to delay cell cycle progression and to induce apoptotic death in several cell lines. Here we report that resveratrol treatment of human glioblastoma cells induces a delay in cell cycle progression during S phase associated with an increase in histone H2AX phosphorylation. Furthermore, with an in vitro assay of topoisomerase IIa catalytic activity we show that resveratrol is able to inhibit the ability of recombinant human TOPO IIa to decatenate kDNA, so that it could be considered a TOPO II poison.
Leone, S., Cornetta, T., Basso, E., Cozzi, R. (2010). Resveratrol induces DNA double strand breaks through human Topoisomerase II interaction. CANCER LETTERS [10.1016/j.canlet.2010.02.022].
Resveratrol induces DNA double strand breaks through human Topoisomerase II interaction
COZZI, Renata
2010-01-01
Abstract
Resveratrol, a stilbene found in grapes and wine, is one of the most interesting natural compound due to its role exerted in cancer prevention and therapy. In particular, resveratrol is able to delay cell cycle progression and to induce apoptotic death in several cell lines. Here we report that resveratrol treatment of human glioblastoma cells induces a delay in cell cycle progression during S phase associated with an increase in histone H2AX phosphorylation. Furthermore, with an in vitro assay of topoisomerase IIa catalytic activity we show that resveratrol is able to inhibit the ability of recombinant human TOPO IIa to decatenate kDNA, so that it could be considered a TOPO II poison.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
