Benvenuti nell'Anagrafe della Ricerca d'Ateneo
La valutazione delle attività di ricerca che si svolgono nelle università ha assunto un'importanza rilevante sia per l'intero sistema universitario sia all'interno di ogni singolo ateneo. Roma Tre si è quindi dotata di idonei strumenti informativi in grado di supportare al meglio le azioni di monitoraggio e di valutazione delle attività di ricerca svolte nei propri dipartimentied ha realizzato una Anagrafe della Ricerca di Ateneo.
EnglishBackground/Aims: In chronic active liver diseases (CALD) with viral aetiology, a population of plasma cells localised in the piecemeal necrosis areas was previously detected by means of autoradiography after in vitro3H-proline incorporation, a method which proved much more sensitive than conventional immunohistochemical procedures. These plasma cells, characteristically located in niches among hepatocytes, in close contact with collagen fibrils, have been hypothesised to exert a role in fibrogenesis stimulation, and particularly in collagen synthesis, possibly through secretion of lymphokines. Specifically, we investigated the presence of interleukin-1, well known to play a crucial role in inflammation and production of collagen by epithelial cells, and to be present in activated plasma cells of myeloma. Methods: The immunohistochemical localisation of interleukin-l/beta in biopsies of patients suffering from chronic active hepatitis was studied, using an affinity purified rabbit polyclonal antibody. Results: The strongest interleukin-l/i immunostaining was observed in the above-described plasma cell population, identified by anti-immunoglobulin antibodies, and 3H-proline incorporation. Conclusions: The ability of plasma cells to produce interleukin-1 during viral CALD suggests that in these pathologies plasma cells play a major role, mainly of paracrine nature. Interleukiu-1, possibly together with other mediators, might in turn stimulate the production of collagen. Hepatocytes of the piecemeal necrosis area appear to be possible candidates for this synthesis, as they show a significant labelling after 3H-proline incorporation, which is absent from hepatocytes far from necrotic areas.
Hassan G, Moreno S, Massimi M, Di Biagio P, & Stefanini S (1997). Interleukin-1-producing plasma cells in close contact with hepatocytes in patients with chronic active hepatitis. JOURNAL OF HEPATOLOGY, 27, 6-17 [10.1016/S0168-8278(97)80273-5].
| Titolo: | Interleukin-1-producing plasma cells in close contact with hepatocytes in patients with chronic active hepatitis | |
| Autori: | ||
| Data di pubblicazione: | 1997 | |
| Rivista: | ||
| Citazione: | Hassan G, Moreno S, Massimi M, Di Biagio P, & Stefanini S (1997). Interleukin-1-producing plasma cells in close contact with hepatocytes in patients with chronic active hepatitis. JOURNAL OF HEPATOLOGY, 27, 6-17 [10.1016/S0168-8278(97)80273-5]. | |
| Abstract: | Background/Aims: In chronic active liver diseases (CALD) with viral aetiology, a population of plasma cells localised in the piecemeal necrosis areas was previously detected by means of autoradiography after in vitro3H-proline incorporation, a method which proved much more sensitive than conventional immunohistochemical procedures. These plasma cells, characteristically located in niches among hepatocytes, in close contact with collagen fibrils, have been hypothesised to exert a role in fibrogenesis stimulation, and particularly in collagen synthesis, possibly through secretion of lymphokines. Specifically, we investigated the presence of interleukin-1, well known to play a crucial role in inflammation and production of collagen by epithelial cells, and to be present in activated plasma cells of myeloma. Methods: The immunohistochemical localisation of interleukin-l/beta in biopsies of patients suffering from chronic active hepatitis was studied, using an affinity purified rabbit polyclonal antibody. Results: The strongest interleukin-l/i immunostaining was observed in the above-described plasma cell population, identified by anti-immunoglobulin antibodies, and 3H-proline incorporation. Conclusions: The ability of plasma cells to produce interleukin-1 during viral CALD suggests that in these pathologies plasma cells play a major role, mainly of paracrine nature. Interleukiu-1, possibly together with other mediators, might in turn stimulate the production of collagen. Hepatocytes of the piecemeal necrosis area appear to be possible candidates for this synthesis, as they show a significant labelling after 3H-proline incorporation, which is absent from hepatocytes far from necrotic areas. | |
| Handle: | http://hdl.handle.net/11590/131798 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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