A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.
Rampioni, G., Bertani, I., Zennaro, E., Polticelli, F., Venturi, V., Leoni, L. (2006). The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter. JOURNAL OF BACTERIOLOGY, 188(2), 815-819 [10.1128/JB.188.2.815-819.2006].
The quorum-sensing negative regulator RsaL of Pseudomonas aeruginosa binds to the lasI promoter
RAMPIONI, Giordano;POLTICELLI, Fabio;LEONI, Livia
2006-01-01
Abstract
A mutation in the rsaL gene of Pseudomonas aeruginosa produces dramatically higher amounts of N-acyl homoserine lactone with respect to the wild type, highlighting the key role of this negative regulator in controlling quorum sensing (QS) in this opportunistic pathogen. The DNA binding site of the RsaL protein on the rsaL-lasI bidirectional promoter partially overlaps the binding site of the LasR protein, consistent with the hypothesis that RsaL and LasR could be in binding competition on this promoter. This is the first direct demonstration that RsaL acts as a QS negative regulator by binding to the lasI promoter.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
