Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H2O2, spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mousemyoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of theSMOandSMOsplice variants is not involved in the differentiation processes.Nuclear localization of only the SMO protein was confirmed.
Cervelli, M., Fratini, E., Amendola, R., Bianchi, M., Signori, E., Ferraro, E., et al. (2009). INCREASED SPERMINE OXIDASE (SMO) ACTIVITY AS A NOVEL DIFFERENTIATION MARKER OF MYOGENIC C2C12 CELLS. THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 41, 934-944 [10.1016/j.biocel.2008.09.009].
INCREASED SPERMINE OXIDASE (SMO) ACTIVITY AS A NOVEL DIFFERENTIATION MARKER OF MYOGENIC C2C12 CELLS
CERVELLI, MANUELA;MARIOTTINI, Paolo
2009-01-01
Abstract
Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H2O2, spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mousemyoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of theSMOandSMOsplice variants is not involved in the differentiation processes.Nuclear localization of only the SMO protein was confirmed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
