The quorum sensing (QS) signalling system of Pseudomonas aeruginosa controls many important functions, including virulence. Although the produc- tion of the QS signal molecule N-3-oxo-dodecanoyl- homoserine lactone (3OC12-HSL) is positively auto- regulated, its concentration reaches a steady level long before stationary phase. The RsaL protein represses transcription of the lasI signal synthase gene, and thus reduces QS signal production. We show that RsaL binds simultaneously with LasR to the rsaL-lasI bidirectional promoter thereby prevent- ing the LasR-dependent activation of both genes. In an rsaL mutant, 3OC12-HSL production continues to increase throughout growth. Thus RsaL provides homeostasis by functioning in opposition to LasR and limiting 3OC12-HSL production to a physiological concentration. Furthermore, transcription profiling revealed that RsaL regulates 130 genes independent of its effect on QS signal molecule production, includ- ing genes involved in virulence. We show that RsaL can repress pyocyanin and hydrogen cyanide viru- lence genes in two ways: directly, by binding to their promoters, and indirectly, by decreasing levels of the signals for their QS signal-dependent transcription. These investigations highlight the importance of RsaL as a global regulator of P. aeruginosa physiology that provides a counterbalance to 3OC12-HSL- dependent gene activation via multiple mechanisms.
|Titolo:||RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa|
LEONI, Livia (Corresponding)
|Data di pubblicazione:||2007|
|Appare nelle tipologie:||1.1 Articolo in rivista|