The occurrence of distinct (2'-5')(A)n-synthetase activities has recently been documented in cytoplasmic and nuclear extracts of several interferon (IFN)-treated cell lines. Since a role has been proposed for (2'-5')(A)n synthetase in the control of cell growth and differentiation, we examined the subcellular distribution of (2'-5')(A)n-synthetase activity both in IFN-treated undifferentiated Friend leukemia cells (FLCs) and during dimethyl-sulfoxide (DMSO)-induced erythroid differentiation of FLCs. Both the nuclear and cytoplasmic (2'-5')(A)n activities were modulated to the same extent by IFNs and DMSO. No evidence for a causal relationship between enzyme activation and FLC differentiation was found.
Mechti, N., Affabris, E., Romeo, G., Lebleu, B. (1985). Subcellular distribution of 2'-5'-oligoadenylate synthetase in differentiating Friend leukemia cells. DIFFERENTIATION, 29(2), 136-139 [10.1111/j.1432-0436.1985.tb00306.x].