"tAtrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in theimmune system. The aim of the present study was to investigate in THP-1 monocytes the ANP effecton hydrogen peroxide (H2O2)-induced Reactive Oxygen Species (ROS), cell proliferation and migration.A significant increase of H2O2-dependent ROS production was induced by physiological concentration ofANP (10−10M). The ANP action was partially affected by cell pretreatment with PD98059, an inhibitor ofmitogen activated-protein kinases (MAPK) as well as by wortmannin, an inhibitor of phosphatidylinositol3-kinase (PI3K) and totally suppressed by diphenylene iodonium (DPI), an inhibitor of the enzyme nico-tinamide adenine dinucleotide phosphate (NADPH) oxidase. The hormone effect was mimicked by cANFand an ANP\/NPR-C signaling pathway was studied using pertussis toxin (PTX). A significant increase ofH2O2-induced cell migration was observed after ANP (10−10M) treatment, conversely a decrease of THP-1proliferation, due to cell death, was found. Both ANP actions were partially prevented by DPI. Moreover,H2O2-induced release of IL-9, TNF-, MIP-1 and MIP-1 was not counteracted by DPI, whereas no effectwas observed in any experimental condition for both IL-6 and IL-1. Our results support the view thatANP can play a key role during the inflammatory process."
Incerpi, S., Affabris, E. (2013). Effect of atrial natriuretic peptide on reactive oxygen species-induced by hydrogen peroxide in THP-1 monocytes: role in cell growth, migration and cytokine release. PEPTIDES, 50, 100-108 [10.1016/j.peptides.2013.09.002].
Effect of atrial natriuretic peptide on reactive oxygen species-induced by hydrogen peroxide in THP-1 monocytes: role in cell growth, migration and cytokine release.
INCERPI, Sandra;AFFABRIS, Elisabetta
2013-01-01
Abstract
"tAtrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in theimmune system. The aim of the present study was to investigate in THP-1 monocytes the ANP effecton hydrogen peroxide (H2O2)-induced Reactive Oxygen Species (ROS), cell proliferation and migration.A significant increase of H2O2-dependent ROS production was induced by physiological concentration ofANP (10−10M). The ANP action was partially affected by cell pretreatment with PD98059, an inhibitor ofmitogen activated-protein kinases (MAPK) as well as by wortmannin, an inhibitor of phosphatidylinositol3-kinase (PI3K) and totally suppressed by diphenylene iodonium (DPI), an inhibitor of the enzyme nico-tinamide adenine dinucleotide phosphate (NADPH) oxidase. The hormone effect was mimicked by cANFand an ANP\/NPR-C signaling pathway was studied using pertussis toxin (PTX). A significant increase ofH2O2-induced cell migration was observed after ANP (10−10M) treatment, conversely a decrease of THP-1proliferation, due to cell death, was found. Both ANP actions were partially prevented by DPI. Moreover,H2O2-induced release of IL-9, TNF-, MIP-1 and MIP-1 was not counteracted by DPI, whereas no effectwas observed in any experimental condition for both IL-6 and IL-1. Our results support the view thatANP can play a key role during the inflammatory process."I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
