Multimeric globins (e.g., hemoglobin) are considered to be the prototypes of allosteric enzymes, whereas monomeric globins (e.g., myoglobin; Mb) usually are assumed to be non-allosteric. However, the modulation of the functional properties of monomeric globins by non-covalent (or allosteric) and covalent modifications casts doubts on this general assumption. Here, we report examples referable to these two extreme mechanisms modulating the reactivity of three mammalian monomeric globins. Sperm whale Mb, which acts as a reserve supply of O-2 and facilitates the O-2 flux within a myocyte, displays the allosteric modulation of the O-2 affinity on lactate, an obligatory product of glycolysis under anaerobic conditions, thus facilitating O-2 diffusion to the mitochondria in supporting oxidative phosphorylation. Human neuroglobin (NGB), which appears to protect neurons from hypoxia in vitro and in vivo, undergoes hypoxia-dependent phosphorylation (i.e., covalent modulation) affecting the coordination equilibrium of the heme-Fe atom and, in turn, the heme-protein reactivity. This facilitates heme-Fe-ligand binding and enhances the rate of anaerobic nitrite reduction to form NO, thus contributing to cellular adaptation to hypoxia. The reactivity of human cytoglobin (CYGB), which has been postulated to protect cells against oxidative stress, depends on both non-covalent and covalent mechanisms. In fact, the heme reactivity of CYGB depends on the lipid, such as oleate, binding which stabilizes the penta-coordination geometry of the heme-Fe atom. Lastly, the reactivity of NGB and CYGB is modulated by the redox state of the intramolecular CysCD7/CysD5 and CysB2/CysE9 residue pairs, respectively, affecting the heme-Fe atom coordination state. In conclusion, the modulation of monomeric globins reactivity by non-covalent and covalent modifications appears a very widespread phenomenon, opening new perspectives in cell survival and protection. This article is part of a Special Issue entitled: Oxygen Binding and Sensing Proteins.
Ascenzi P, Marino M, Polticelli F, Coletta M, Gioia M, Marini S, et al. (2013). Non-covalent and covalent modifications modulate the reactivity of monomeric mammalian globins. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834(9), 1750-1756 [10.1016/j.bbapap.2013.02.012].
|Titolo:||Non-covalent and covalent modifications modulate the reactivity of monomeric mammalian globins|
|Data di pubblicazione:||2013|
|Citazione:||Ascenzi P, Marino M, Polticelli F, Coletta M, Gioia M, Marini S, et al. (2013). Non-covalent and covalent modifications modulate the reactivity of monomeric mammalian globins. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834(9), 1750-1756 [10.1016/j.bbapap.2013.02.012].|
|Appare nelle tipologie:||1.1 Articolo in rivista|