"A huge amount of experimental data demonstrated that the natural compound Resveratrol (RESV) exerts various beneficial effects on human health. Controversial data are present in literature about the ability of RESV to affect DNA molecule integrity. Since controlled exposure to ionising radiation (IR) is one of the most effective treatments of cancer patients we focused our attention on a possible radioprotective activity of RESV in normal tissues. We investigated the ability of RESV to modulate the chromosome damage induced by IR using the Cytokinesis-Block Micronucleus (CBMN) assay and the Chromosomal Aberration (CA) test on human peripheral blood lymphocytes. While RESV by itself does not induce any DNA damage, we surprisingly found a controversial effect in combined treatment: a reduction of IR-induced MN and an increase of IR-induced CA. These results suggested a deeper analysis of the influence of RESV both on DNA repair efficiency and on cell viability. The modulation by RESV of DNA repair has been analysed through Comet assay and γ-H2AX foci induction. The influence of RESV on cell viability has been assessed through apoptosis and cell growth analysis."
Basso, E., Palma, V., Leone, S., Testa, A., Cozzi, R. (2012). Does Resveratrol affect radiation-induced chromosome damage? A multiparametric study on human lymphocytes exposed in vitro.
Does Resveratrol affect radiation-induced chromosome damage? A multiparametric study on human lymphocytes exposed in vitro
BASSO, EMILIANO;COZZI, Renata
2012-01-01
Abstract
"A huge amount of experimental data demonstrated that the natural compound Resveratrol (RESV) exerts various beneficial effects on human health. Controversial data are present in literature about the ability of RESV to affect DNA molecule integrity. Since controlled exposure to ionising radiation (IR) is one of the most effective treatments of cancer patients we focused our attention on a possible radioprotective activity of RESV in normal tissues. We investigated the ability of RESV to modulate the chromosome damage induced by IR using the Cytokinesis-Block Micronucleus (CBMN) assay and the Chromosomal Aberration (CA) test on human peripheral blood lymphocytes. While RESV by itself does not induce any DNA damage, we surprisingly found a controversial effect in combined treatment: a reduction of IR-induced MN and an increase of IR-induced CA. These results suggested a deeper analysis of the influence of RESV both on DNA repair efficiency and on cell viability. The modulation by RESV of DNA repair has been analysed through Comet assay and γ-H2AX foci induction. The influence of RESV on cell viability has been assessed through apoptosis and cell growth analysis."I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
