Qualitative and Quantitative Validation of the Micro Biological Survey Method for Listeria spp., Salmonella spp., Enterobactericeae and Staphylococcus aureus in Food Samples

""The objective of the present study was the preliminary validation in accord with ISO 16140 (2003) of the qualitative MBS methods for Salmonella spp. and Listeria spp. and the quantitative MBS methods for Enterobacteriaceae and Staphylococcus aureus. The MBS method is a rapid alternative method for the detection and selective counting of bacteria in agro-food, in water and in environmental samples. It is based on colorimetric survey in mono-use disposable reaction vials that must be filled with the samples without any preliminary treatment (eg. homogenization, dilution, etc.); the greater the number of bacteria present in the sample, the faster the color change. For the qualitative validation of the MBS methods for Salmonella spp. and Listeria spp., selectivity, relative accuracy, relative specificity and relative sensitive were determined. Selectivity, linearity and accuracy were analyzed for the quantitative validation of the MBS methods for Enterobacteriaceae and Staphylococcus aureus. The validation here reported has shown that the MBS methods for Listeria spp., Salmonella spp., Enterobacteriaceae and S. aureus gave similar results and were in agreement with the reference methods.""