Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples

""The rom of this study was the validation of the Micro Biological Survey (MBS) method for. microbiological analysis of food for Total Viable Count (TVC) and Escherichia coli (E. coli), The. MBS method is a rapid quantitative alternative method for the detection and selective counting of. bacteria in agro-food, in water and in environmental samples. It is based on colorimetric survey in. mono-uss disposable reaction vials that must be filled with the samples without any preliminary. treatment (e.g., homogenization, dilution, etc.); the greater the number ofbacteria presents into. the sample, the faster the color change. However, an independent evaluation of the analytical. results obt.ained with MBS method would be required before commercialization. Therefore, this. alternative method was validated in comparison to the reference method. The general estimate of. precision, reliability, uncertainty, linearity, accuracy and selectivity were determined. Ali the. performance parameters have demonstrated total corrslation between the alternative method and. the reference method for the detection and counting of TVC and E. coli both in artificially. contaminated and in naturaliy contaminated samples. MBS assay can be used as rapid and user. friendly screening method for detection of TVC and E. coli in food industry.""