Treatment of leukemia cells with 1, 25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.
Fontemaggi, G., Bellissimo, T., Donzelli, S., Iosue, I., Benassi, B., Bellotti, G., et al. (2015). Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition. RNA BIOLOGY, 12(7), 690-700 [10.1080/15476286.2015.1044194].
Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition
BELLOTTI, GIORGIO;
2015-01-01
Abstract
Treatment of leukemia cells with 1, 25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
