Different in vitro response to rIL-1β of newborn and adult rat astroglia

http://sfx.cilea.it:9003/sfxcab3/sf(opens in a new window)|View at Publisher| Export | Download | Save to list | More... International Journal of Developmental Neuroscience Volume 9, Issue 5, 1991, Pages 501-507 Different in vitro response to rIL-1β of newborn and adult rat astroglia (Article) Colasanti, M.a, Ramacci, M.T.b, Foresta, P.c, Lauro, G.M.a a Dipartimento di Biologia Cellulare e dello Sviluppo, Università La Sapienza, Via degli Apuli, 1-00185 Roma, Italy b Istituto per la Ricerca sulla Senescenza. SIGMA-TAU, Pomezia, Italy View references (17) Abstract In recent literature, lymphokines have been reported to be able to promote both proliferation and maturation of some glial populations. In this paper, we compare the effect of rIL-1 on newborn and adult rat astroglial cells in vitro. In newborn, but not in adult astrocytes, 100 U/ml of rIL-1β increase [3H]thymidine incorporation with a maximal response by 3 days as compared to the control untreated culture. In contrast, rIL-1β induces an increase of GFAP immunoreactivity both in newborn and in adult astrocytes, as compared to the control untreated cells. These data indicate that, while both newborn and adult astroglial cells are capable of responding to rIL-1β, only newborn astrocytes can respond to this lymphokine with proliferation. Thus, it appears likely that different factors, other than rIL-1β, are needed by adult astrocytes to proliferate.