Lipid A aminoarabinosylation is invariably associated with colistin resistance inPseudomonas aeruginosa; however, the existence of alternative, aminoarabinosylation-independent colistin resistance mechanisms in this bacterium remained elusive. By combining reverse genetics with experimental evolution assays we demonstrate that a functional lipid A aminoarabinosylation pathway is critical for acquisition of colistin resistance in reference and clinicalP. aeruginosaisolates. This highlights lipid A aminoarabinosylation as a promising target for the design of colistin adjuvants againstP. aeruginosa.
LO SCIUTO, A., Imperi, F. (2018). Aminoarabinosylation of lipid A is critical for the development of colistin resistance in Pseudomonas aeruginosa. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AAC.01820-17 [10.1128/AAC.01820-17].
Aminoarabinosylation of lipid A is critical for the development of colistin resistance in Pseudomonas aeruginosa
Imperi, Francesco
2018-01-01
Abstract
Lipid A aminoarabinosylation is invariably associated with colistin resistance inPseudomonas aeruginosa; however, the existence of alternative, aminoarabinosylation-independent colistin resistance mechanisms in this bacterium remained elusive. By combining reverse genetics with experimental evolution assays we demonstrate that a functional lipid A aminoarabinosylation pathway is critical for acquisition of colistin resistance in reference and clinicalP. aeruginosaisolates. This highlights lipid A aminoarabinosylation as a promising target for the design of colistin adjuvants againstP. aeruginosa.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.