Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated by ABA and that two Atcuaoδ T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2 O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2 O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAOδ over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2 O2 production compared to WT plants. Our data suggest that AtCuAOδ is involved in the H2 O2 production related to ABA-induced stomatal closure.

Fraudentali, I., Ghuge, S.A., Carucci, A., Tavladoraki, P., Angelini, R., Cona, A., et al. (2019). The copper amine oxidase AtCuAOδ participates in abscisic acid-induced stomatal closure in arabidopsis. PLANTS, 8(6), 183 [10.3390/plants8060183].

The copper amine oxidase AtCuAOδ participates in abscisic acid-induced stomatal closure in arabidopsis

Fraudentali I.;Ghuge S. A.;Carucci A.;Tavladoraki P.;Angelini R.;Cona A.;
2019-01-01

Abstract

Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated by ABA and that two Atcuaoδ T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2 O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2 O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAOδ over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2 O2 production compared to WT plants. Our data suggest that AtCuAOδ is involved in the H2 O2 production related to ABA-induced stomatal closure.
Fraudentali, I., Ghuge, S.A., Carucci, A., Tavladoraki, P., Angelini, R., Cona, A., et al. (2019). The copper amine oxidase AtCuAOδ participates in abscisic acid-induced stomatal closure in arabidopsis. PLANTS, 8(6), 183 [10.3390/plants8060183].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11590/355695
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