Benvenuti nell'Anagrafe della Ricerca d'Ateneo
La valutazione delle attività di ricerca che si svolgono nelle università ha assunto un'importanza rilevante sia per l'intero sistema universitario sia all'interno di ogni singolo ateneo. Roma Tre si è quindi dotata di idonei strumenti informativi in grado di supportare al meglio le azioni di monitoraggio e di valutazione delle attività di ricerca svolte nei propri dipartimentied ha realizzato una Anagrafe della Ricerca di Ateneo.
EnglishObjective: Glioblastoma (GBM) is the most aggressive and common form of primary brain cancer. Survival is poor and improved treatment options are urgently needed. Dual specificity phosphatase-6 (DUSP6) is actively involved in oncogenesis showing unexpected tumor-promoting properties in human glioblastoma, contributing to the development and expression of the full malignant and invasive phenotype. The purpose of this study was to assess if DUSP6 activates epithelial-to-mesenchymal transition (EMT) in glioblastoma and its connection with the invasive capacity. Results: We found high levels of transcripts mRNA by qPCR analysis in a panel of primary GBM compared to adult or fetal normal tissues. At translational levels, these data correlate with high protein expression and long half-life values by cycloheximide-chase assay in immunoblot experiments. Next, we demonstrate that DUSP6 gene is involved in epithelial-to-mesenchymal transition (EMT) in GBM by immunoblot characterization of the mesenchymal and epithelial markers. Vimentin, N-Cadherin, E-Cadherin and fibronectin were measured with and without DUSP6 over-expression, and in response to several stimuli such as chemotherapy treatment. In particular, the high levels of vimentin were blunted at increasing doses of cisplatin in condition of DUSP6 over-expression while N-Cadherin contextually increased. Finally, DUSP6 per se increased invasion capacity of GBM. Overall, our data unveil the DUSP6 involvement in invasive mesenchymal-like properties in GBM.
Zuchegna, C., Di Zazzo, E., Moncharmont, B., & Messina, S. (2020). Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement. BMC RESEARCH NOTES, 13(1), 374.
| Titolo: | Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement |
| Autori: | Messina, Samantha (Corresponding) |
| Data di pubblicazione: | 2020 |
| Rivista: | |
| Citazione: | Zuchegna, C., Di Zazzo, E., Moncharmont, B., & Messina, S. (2020). Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement. BMC RESEARCH NOTES, 13(1), 374. |
| Handle: | http://hdl.handle.net/11590/378240 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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