Objective: Glioblastoma (GBM) is the most aggressive and common form of primary brain cancer. Survival is poor and improved treatment options are urgently needed. Dual specificity phosphatase-6 (DUSP6) is actively involved in oncogenesis showing unexpected tumor-promoting properties in human glioblastoma, contributing to the development and expression of the full malignant and invasive phenotype. The purpose of this study was to assess if DUSP6 activates epithelial-to-mesenchymal transition (EMT) in glioblastoma and its connection with the invasive capacity. Results: We found high levels of transcripts mRNA by qPCR analysis in a panel of primary GBM compared to adult or fetal normal tissues. At translational levels, these data correlate with high protein expression and long half-life values by cycloheximide-chase assay in immunoblot experiments. Next, we demonstrate that DUSP6 gene is involved in epithelial-to-mesenchymal transition (EMT) in GBM by immunoblot characterization of the mesenchymal and epithelial markers. Vimentin, N-Cadherin, E-Cadherin and fibronectin were measured with and without DUSP6 over-expression, and in response to several stimuli such as chemotherapy treatment. In particular, the high levels of vimentin were blunted at increasing doses of cisplatin in condition of DUSP6 over-expression while N-Cadherin contextually increased. Finally, DUSP6 per se increased invasion capacity of GBM. Overall, our data unveil the DUSP6 involvement in invasive mesenchymal-like properties in GBM.
Zuchegna, C., Di Zazzo, E., Moncharmont, B., & Messina, S. (2020). Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement. BMC RESEARCH NOTES, 13(1), 374.
Titolo: | Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement |
Autori: | Messina, Samantha (Corresponding) |
Data di pubblicazione: | 2020 |
Rivista: | |
Citazione: | Zuchegna, C., Di Zazzo, E., Moncharmont, B., & Messina, S. (2020). Dual-specificity phosphatase (DUSP6) in human glioblastoma: Epithelial-to-mesenchymal transition (EMT) involvement. BMC RESEARCH NOTES, 13(1), 374. |
Handle: | http://hdl.handle.net/11590/378240 |
Appare nelle tipologie: | 1.1 Articolo in rivista |