The tripeptide Glycyl-L-prolyl-glycinamide HCl (GPG-NH2) is chosen as a model system to investigate beta-turns formation in aqueous solutions and the role of trehalose in preserving this motif in drought conditions. In this first study relatively dilute solutions are investigated. Neutron diffraction experiments show the presence of both direct and water mediated b-turns, the latter ones increasing in number when trehalose is added to the aqueous solution. The structure of the ternary (GPG-NH2/water/trehalose) solution demonstrates that trehalose confines water close to the b-turns, possibly preserving their hydration when water runs low, without directly interacting with GPG-NH2 via Hydrogen bonds. Further experiments at lower water content are needed to confirm this finding.

Di Gioacchino, M., Bruni, F., Ricci, M.A. (2021). GPG-NH2 solutions: A model system for β-turns formation. Possible role of trehalose against drought. JOURNAL OF MOLECULAR LIQUIDS, 335, 116514 [10.1016/j.molliq.2021.116514].

GPG-NH2 solutions: A model system for β-turns formation. Possible role of trehalose against drought

Di Gioacchino, M.
Membro del Collaboration Group
;
Bruni, F.
Membro del Collaboration Group
;
Ricci, M. A.
Membro del Collaboration Group
2021-01-01

Abstract

The tripeptide Glycyl-L-prolyl-glycinamide HCl (GPG-NH2) is chosen as a model system to investigate beta-turns formation in aqueous solutions and the role of trehalose in preserving this motif in drought conditions. In this first study relatively dilute solutions are investigated. Neutron diffraction experiments show the presence of both direct and water mediated b-turns, the latter ones increasing in number when trehalose is added to the aqueous solution. The structure of the ternary (GPG-NH2/water/trehalose) solution demonstrates that trehalose confines water close to the b-turns, possibly preserving their hydration when water runs low, without directly interacting with GPG-NH2 via Hydrogen bonds. Further experiments at lower water content are needed to confirm this finding.
Di Gioacchino, M., Bruni, F., Ricci, M.A. (2021). GPG-NH2 solutions: A model system for β-turns formation. Possible role of trehalose against drought. JOURNAL OF MOLECULAR LIQUIDS, 335, 116514 [10.1016/j.molliq.2021.116514].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11590/388370
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