This study focuses on understanding how growing iPSCs on different ECM coating substrates can affect cell confluence. A protocol to assess iPSC confluence in real time has been established without the need to count cells in single cell suspension to avoid any growth perturbation. A high-content image analysis system was used to assess iPCS confluence on 4 different ECMs over time in an automated manner. Different analysis settings were used to assess cell confluence of adherent iPSCs and only a slight difference (at 24 and 48 hours with laminin) has been observed whether a 60, 80 or 100% mask was applied. We also show that laminin lead to the best confluence compared to Matrigel, vitronectin and fibronectin.
Magliocca, V., Vinci, M., Persichini, T., Locatelli, F., Tartaglia, M., & Compagnucci, C. (2020). Measuring the confluence of iPSCs using an automated imaging system. JOURNAL OF VISUALIZED EXPERIMENTS, 2020(160), 1-10 [10.3791/61225].
Titolo: | Measuring the confluence of iPSCs using an automated imaging system | |
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Data di pubblicazione: | 2020 | |
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Citazione: | Magliocca, V., Vinci, M., Persichini, T., Locatelli, F., Tartaglia, M., & Compagnucci, C. (2020). Measuring the confluence of iPSCs using an automated imaging system. JOURNAL OF VISUALIZED EXPERIMENTS, 2020(160), 1-10 [10.3791/61225]. | |
Handle: | http://hdl.handle.net/11590/395859 | |
Appare nelle tipologie: | 1.1 Articolo in rivista |