Antiretroviral therapy allows a restoration of immune cell homeostasis associated with a normal immune competence. Our goal was to analyze the modulation of polyfunctional HIV-specific CD8+ T-cell responses during antiretroviral therapy. HIV-infected individuals were divided into four groups according to CD4+ cell count and viral load at the moment of recruitment. Whole blood was stimulated with a pool of CD8-specific HIV-antigens to assess cytokine/chemokine production and cytotoxicity activity by using flow cytometry. The groups show different modulation in HIV-specific CD8+ T-cell responses. In particular, immunological failure showed different distributions of polyfunctional HIV-specific CD8+ responses, mainly due to an increase of cells producing CD107a/IFNγ/IL-2/MIP-1β. Our results indicate that this particular 4+ functional subset is a possible correlate of immunological failure. Considering the complexity of interactions among HAART, immune system and HIV, work is in progress to find correlates of therapy efficacy. Copyright © by BIOLIFE, s.a.s.
Casetti, R., De Simone, G., Sacchi, A., Bordoni, V., Viola, D., Rinaldi, A., et al. (2014). Modulation of polyfunctional HIV-specific CD8 T cells in patients responding differently to antiretroviral therapy. INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY, 27(2), 291-297 [10.1177/039463201402700218].
Modulation of polyfunctional HIV-specific CD8 T cells in patients responding differently to antiretroviral therapy
Sacchi A.;
2014-01-01
Abstract
Antiretroviral therapy allows a restoration of immune cell homeostasis associated with a normal immune competence. Our goal was to analyze the modulation of polyfunctional HIV-specific CD8+ T-cell responses during antiretroviral therapy. HIV-infected individuals were divided into four groups according to CD4+ cell count and viral load at the moment of recruitment. Whole blood was stimulated with a pool of CD8-specific HIV-antigens to assess cytokine/chemokine production and cytotoxicity activity by using flow cytometry. The groups show different modulation in HIV-specific CD8+ T-cell responses. In particular, immunological failure showed different distributions of polyfunctional HIV-specific CD8+ responses, mainly due to an increase of cells producing CD107a/IFNγ/IL-2/MIP-1β. Our results indicate that this particular 4+ functional subset is a possible correlate of immunological failure. Considering the complexity of interactions among HAART, immune system and HIV, work is in progress to find correlates of therapy efficacy. Copyright © by BIOLIFE, s.a.s.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
