DNA polymerase III (Pol III) is the replicative enzyme in bacteria. It consists of three sub-complexes, the catalytic core, the β clamp, and the clamp loader. While this complex has been thoroughly characterized in the model organism Escherichia coli, much less is known about its functioning and/or its specific properties in other bacteria. Biochemical studies highlighted specific features in the clamp loader subunit ψ of Pseudomonas aeruginosa as compared to its E. coli counterpart, and transposon mutagenesis projects identified the ψ-encoding gene holD among the strictly essential core genes of P. aeruginosa. By generating a P. aeruginosa holD conditional mutant, here we demonstrate that, as previously observed for E. coli holD mutants, HolD-depleted P. aeruginosa cells show strongly decreased growth, induction of the SOS response, and emergence of suppressor mutants at high frequency. However, differently from what was observed in E. coli, the growth of P. aeruginosa cells lacking HolD cannot be rescued by the deletion of genes for specialized DNA polymerases. We also observed that the residual growth of HolD-depleted cells is strictly dependent on homologous recombination functions, suggesting that recombination-mediated rescue of stalled replication forks is crucial to support replication by a ψ-deficient Pol III enzyme in P. aeruginosa.
Spinnato, M.C., Sciuto, A.L., Mercolino, J., Lucidi, M., Leoni, L., Rampioni, G., et al. (2022). Effect of a Defective Clamp Loader Complex of DNA Polymerase III on Growth and SOS Response in Pseudomonas aeruginosa. MICROORGANISMS, 10(2), 423 [10.3390/microorganisms10020423].
Titolo: | Effect of a Defective Clamp Loader Complex of DNA Polymerase III on Growth and SOS Response in Pseudomonas aeruginosa | |
Autori: | IMPERI, FRANCESCO (Corresponding) | |
Data di pubblicazione: | 2022 | |
Rivista: | ||
Citazione: | Spinnato, M.C., Sciuto, A.L., Mercolino, J., Lucidi, M., Leoni, L., Rampioni, G., et al. (2022). Effect of a Defective Clamp Loader Complex of DNA Polymerase III on Growth and SOS Response in Pseudomonas aeruginosa. MICROORGANISMS, 10(2), 423 [10.3390/microorganisms10020423]. | |
Handle: | http://hdl.handle.net/11590/401920 | |
Appare nelle tipologie: | 1.1 Articolo in rivista |
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